Flow Cytometry data from the R/V TINRO, NOAA Bell M. Shimada and CCGS Sir John Franklin during the 2022 International Year of the Salmon Pan-Pacific Winter High Seas Expedition

Phytoplankton community composition and size structure vary considerably between oligotrophic and eutrophic regions (areas of low or high macro and micronutrients (e.g., iron)) (Hill et al., 2005; Martin et al., 1989; Strom et al., 2006; 2016), between surface and subsurface depths (Hill et al., 2005), and with season (Moran et al., 2012) and climatic conditions (Batten et al., 2021). Phytoplankton represent the base of the food web providing energy for zooplankton, which in turn support the growth of juvenile and adult salmon populations. Certain phytoplankton, like many diatom species, are particularly important food items in the GOA (Odate, 1996, Strom et al., 2007). We aim to broaden understanding of phytoplankton dynamics in the GOA/NPO by investigating spatial and temporal patterns in community structure and biomass and exploring environmental (physics and nutrients) drivers of taxonomic variability that may lead to variation in the quality of phytoplankton biomass available to primary consumers during winter. The spatial variations in phytoplankton biomass, and taxa and community size structure were characterized through measurements of total chla, flow cytometry, and HPLC (analyzed by Canadian PIs Konik, Costa) collected at all IYS stations. In zone 4 (US Ship Shimada) flow cytometry samples were collected from 5, 25, and 50 m for assessment of microbial community cell sizes (Moran et al. 2012), and HPLC samples from 5 m for assessment of phytoplankton functional groups; both data sets will be used to help ground-truth the information obtained from the satellite ocean color measurements. Flow cytometry and HPLC samples were also collected at 5 m depths in zone 5 (on Canadian ship Franklin) and in zones 2-3 (on Russian ship TINRO), so we have samples over the entire area surveyed. The flow cytometry analysis deliverables include tabulated counts (cells/ml) and estimated carbon content (C/cell and C/population) for the following 4 phytoplankton pico- and nanoplankton: Synechococcus, Cryptophytes, picoeukaryotes, nanoeukaryotes (excluding Cryptophytes). Water samples for total chlorophyll a (Chla), were collected from Niskin bottles at six depths (5, 25, 50, 75, 100, 150 m) at all stations surveyed.

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Licence: Creative Commons Attribution 4.0

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Metadata Reference Date(s) August 16, 2022 (Publication)
September 08, 2022 (Revision)
Dataset Reference Date(s) February 05, 2022 (Creation)
Frequency of Update As Needed

Responsible Party 1
Name
Eisner, Lisa B.
Affiliation
National Oceanic and Atmospheric Administration
Email
lisa.eisner@noaa.gov
Role
  • Author
  • Custodian
  • Owner
  • Principal Investigator
Responsible Party 2
Name
Lomas, Mike W.
Affiliation
Bigelow Laboratory for Ocean Sciences
Email
mlomas@bigelow.org
Role
  • Author
  • Processor
Responsible Party 3
Affiliation
North Pacific Anadromous Fish Commission
Role
Publisher
Responsible Party 4
Affiliation
Hakai Institute
Role
Distributor

Field Value
Ocean Variables
  • Phytoplankton biomass and diversity
  • Other
Scope Dataset
Status Completed
Maintenance Note Generated from https://cioos-siooc.github.io/metadata-entry-form
Spatial Extent { "coordinates": [ [ [ -172.628, 44.996 ], [ -126.401, 44.996 ], [ -126.401, 58.009 ], [ -172.628, 58.009 ], [ -172.628, 44.996 ] ] ], "type": "Polygon" }
North Bounding Latitude 58.009
South Bounding Latitude 44.996
East Bounding Longitude -126.401
West Bounding Longitude -172.628
Temporal Extent
Begin
2022-02-05
End
2022-03-20
Vertical Extent
Min
0.0
Max
5.0
Default Locale English
Citation identifier
Code
10.21966/j26w-by50